GMP 3T3 Fibroblast Cells

Waisman Biomanufacturing offers irradiated 3T3 feeder cells produced and tested according to cGMP guidelines. These cells will be provided to researchers on a non-profit basis under a contract from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) to support skin gene therapy and wound healing clinical trials. 3T3 cells are a murine derived fibroblast cell line that is used as feeder cells for growing keratinocytes for a variety of applications.

Each vial contains a minimum of 3 X 106 cells that have been manufactured in fully compliant cleanrooms, and are certified mycoplasma free and low endotoxin. In addition, the cell bank has been fully tested for adventitious agents, including murine viruses. These cells have been screened for keratinocyte culture support and do not contain mitomycin C. 


3T3 fibroblasts are used as feeder cells in culturing human keratinocytes for a variety of uses including skin gene therapy, skin grafts, and wound healing research.

Product Specifications

• Cells per vial: >3x106
• Plating Efficiency: 1 vial will seed a P100 culture dish to > 30% confluence
• Endotoxin: < 10 EU /ml
• Sterility (as per 21 CFR 610.12): No growth
• Mycoplasma: Negative
• Cryopreservation Media: 10-20% bovine serum and 10% DMSO in DMEM (1 ml)

Vial Contents: >3x106 cells

Order Information: Please contact Brian Dattilo, Manager of Business Development.


To order please download and complete a Cell Request form and a User-Waisman Biomanufacturing Material Transfer Agreement (MTA).
3T3 Cell Request Form
• User-Waisman Material Transfer Agreement

The links below are agreements made between Waisman Biomanufacturing and the original cell line sources as referenced in the above MTA.

NIH Cell Line 
Waisman MTA J2 Cell Line


  • Todaro GJ, Green H. Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines. J. Cell Biol. 17: 299-313, 1963. PubMed: 13985244
  • Weterings PJ, Roelofs HM, Vermorken AJ, Bloemendal H. Serial cultivation of human scalp hair follicle keratinocytes. Acta Derm Venereol 63(4):315-20. 1983. PubMed: 6195863