GMP rhFGF-basic (155 aa)

Waisman Biomanufacturing sells human FGF-basic (synonyms: FGF-basic, bFGF, FGFb, hFGF-2) produced under cGMP conditions for use in culturing stem cells for human clinical trials or in research applications that are anticipated to lead to clinical research.

Quality Specifications

Vialed product undergoes extensive testing to demonstrate that it meets strict requirements for identity, purity, and potency. In addition to standard assays to monitor the overall purity of the product, two cell culture potency assays are performed on each lot:  MTT and Human ES Cell Proliferation Assays. The Human ES Cell Proliferation Assay consists of culturing human ES cells (WA09) for five passages using feeder-independent culture conditions (TeSR media, 100 ng/mL rhFGF2). At the end of five passages, the human ES cells are assessed for proliferation, colony morphology and differentiation based on flow cytometry analysis of human ES cell marker expression (> 85% Oct4/SSEA4 double positive).


Product Specifications

  • Purity (by HPLC):  Total Purity ≥ 90%, Monomer Purity ≥ 75%
  • Protein Concentration by UV:  0.10 – 0.13 mg/mL
  • Fill Volume:  1.1 +/- 0.1 mL
  • SDS-PAGE:  Compares favorably to standard
  • Sterility:  No Growth
  • Endotoxin:  < 0.1EU/µg
  • Activity (MTT assay) EC50:  < 2.0 ng/mL
  • Human ES Cell Proliferation Assay:  Supports growth of undifferentiated human ES cells
Vial contents: 100 µg/vial, 0.1 mg/mL in aqueous buffer
cGMP Grade $189/vial (plus S&H)
Research Grade $94.50/vial (plus S&H)

Order Information:  Please contact Kayla Schmeling.


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