Services and Products
GMP rhFGF-basic (155 aa)
Waisman Biomanufacturing sells human FGF-basic (synonyms: FGF-basic, bFGF, FGFb, hFGF-2) produced under CGMP compliance for use as a raw material or ancillary agent in culturing stem cells for human clinical trials.
Quality Specifications
Bulk and vialed product undergoes extensive testing to demonstrate that it meets strict requirements for identity, purity, and strength. In addition to standard assays to monitor the overall purity of the product, a Human ES Cell Proliferation Assay is performed to demonstrate the potency of the product. The Human ES Cell Proliferation Assay consists of culturing human ES cells (WA09) for five passages using feeder-independent culture conditions (TeSR media, 100 ng/mL rhFGF2). At the end of five passages, the human ES cells are assessed for proliferation, colony morphology and differentiation based on flow cytometry analysis of human ES cell marker expression (> 85% Oct4/SSEA4 double positive).
Product Specifications
- Purity (by HPLC): Total Purity ≥ 90%, Monomer Purity ≥ 75%
- Protein Concentration by UV: 0.10 – 0.13 mg/mL
- Fill Volume: 1.1 +/- 0.1 mL
- SDS-PAGE: Compares favorably to standard
- Sterility: No Growth
- Endotoxin: < 0.1EU/µg
- Human ES Cell Proliferation Assay: Supports growth of undifferentiated human ES cells
Custom Packaging Available: Injection grade ISO2R/6R/10R glass vial contents 0.1-10.0 mL; PETG bottle contents 5-200 mL; custom protein concentrations.
Order Information: Only large batch custom orders are available. Please contact for more information.
Email: info@gmpbio.org.
References
- Beenken, A. and Mohammadi, M. (2009). The FGF family: biology, pathophysiology and therapy. Nature Reviews Drug Discovery 8, 235-253. PubMed 19247306
- Caldwell,M.A. and Svendsen,C.N. (1998). Heparin, but not other proteoglycans potentiates the mitogenic effects of FGF-2 on mesencephalic precursor cells. Experimental Neurology 152, 1-10. PubMed 9682007
- Kashiwakura,I. and Takahashi,T.A. (2004). Fibroblast growth factor and ex vivo expansion of hematopoietic progenitor cells. Leukemia & Lymphoma 46, 329-333. PubMed 15621822
- Lee,S.Y., Lim,J., Khang,G., Son,Y., Choung,P.H., Kang,S.S., Chun,S.Y., Shin,H.I., Kim,S.Y., and Park,E.K. (2009). Enhanced Ex Vivo Expansion of Human Adipose Tissue-Derived Mesenchymal Stromal Cells by Fibroblast Growth Factor-2 and Dexamethasone. Tissue Engineering Part A 15, 2491-2499. PubMed 19292683
- Levenstein,M.E., Ludwig,T.E., Xu,R.H., Llanas,R.A., VanDenHeuvel-Kramer,K., Manning,D., and Thomson,J.A. (2006). Basic fibroblast growth factor support of human embryonic stem cell self-renewal. Stem Cells 24, 568-574. PubMed 16282444
- Ludwig,T.E., Levenstein,M.E., Jones,J.M., Berggren,W.T., Mitchen,E.R., Frane,J.L., Crandall,L.J., Daigh,C.A., Conard,K.R., Piekarczyk,M.S., Llanas,R.A., and Thomson,J.A. (2006). Derivation of human embryonic stem cells in defined conditions. Nature Biotechnology 24, 185-187. PubMed 16388305