GMP rhFGF-basic (155 aa)

Waisman Biomanufacturing sells human FGF-basic (synonyms: FGF-basic, bFGF, FGFb, hFGF-2) produced under cGMP conditions for use in culturing stem cells for human clinical trials or in research applications that are anticipated to lead to clinical research.

Quality Specifications

Vialed product undergoes extensive testing to demonstrate that it meets strict requirements for identity, purity, and potency. In addition to standard assays to monitor the overall purity of the product, two cell culture potency assays are performed on each lot:  MTT and Human ES Cell Proliferation Assays. The Human ES Cell Proliferation Assay consists of culturing human ES cells (WA09) for five passages using feeder-independent culture conditions (TeSR media, 100 ng/mL rhFGF2). At the end of five passages, the human ES cells are assessed for proliferation, colony morphology and differentiation based on flow cytometry analysis of human ES cell marker expression (> 85% Oct4/SSEA4 double positive).

 

Product Specifications

  • Purity (by HPLC):  Total Purity ≥ 90%, Monomer Purity ≥ 75%
  • Protein Concentration by UV:  0.10 – 0.13 mg/mL
  • Fill Volume:  1.1 +/- 0.1 mL
  • SDS-PAGE:  Compares favorably to standard
  • Sterility:  No Growth
  • Endotoxin:  < 0.1EU/µg
  • Activity (MTT assay) EC50:  < 2.0 ng/mL
  • Human ES Cell Proliferation Assay:  Supports growth of undifferentiated human ES cells
 
Vial contents: 100 µg/vial, 0.1 mg/mL in aqueous buffer
cGMP Grade $189/vial (plus S&H)
Research Grade $94.50/vial (plus S&H)

Order Information:  Please contact Kayla Schmeling.
Email: kschmeling@waisman.wisc.edu
 

References

  • Beenken, A. and Mohammadi, M. (2009). The FGF family: biology, pathophysiology and therapy. Nature Reviews Drug Discovery 8, 235-253. PubMed 19247306
  • Caldwell,M.A. and Svendsen,C.N. (1998). Heparin, but not other proteoglycans potentiates the mitogenic effects of FGF-2 on mesencephalic precursor cells. Experimental Neurology 152, 1-10. PubMed 9682007
  • Kashiwakura,I. and Takahashi,T.A. (2004). Fibroblast growth factor and ex vivo expansion of hematopoietic progenitor cells. Leukemia & Lymphoma 46, 329-333. PubMed 15621822
  • Lee,S.Y., Lim,J., Khang,G., Son,Y., Choung,P.H., Kang,S.S., Chun,S.Y., Shin,H.I., Kim,S.Y., and Park,E.K. (2009). Enhanced Ex Vivo Expansion of Human Adipose Tissue-Derived Mesenchymal Stromal Cells by Fibroblast Growth Factor-2 and Dexamethasone. Tissue Engineering Part A 15, 2491-2499. PubMed 19292683
  • Levenstein,M.E., Ludwig,T.E., Xu,R.H., Llanas,R.A., VanDenHeuvel-Kramer,K., Manning,D., and Thomson,J.A. (2006). Basic fibroblast growth factor support of human embryonic stem cell self-renewal. Stem Cells 24, 568-574. PubMed 16282444
  • Ludwig,T.E., Levenstein,M.E., Jones,J.M., Berggren,W.T., Mitchen,E.R., Frane,J.L., Crandall,L.J., Daigh,C.A., Conard,K.R., Piekarczyk,M.S., Llanas,R.A., and Thomson,J.A. (2006). Derivation of human embryonic stem cells in defined conditions. Nature Biotechnology 24, 185-187. PubMed 16388305